43. ALICE-LOW-FUNG MUI
Information acquired: 2025
ALICE-LOW-FUNG MUI is an academic who published a paper in 1992 entitled Interleukin-3 signal transduction : purification and characterization of the murine interleukin-3 re. ALICE-LOW-FUNG MUI published this work as part of a team: Mui, Alice Low-Fung. Here is a description of this work: Murine interleukin-3 (mIL-3) is a potent hemopoietic growth factor that is produced primarily by activated T lymphocytes and stimulates the proliferation and differentiation of pluripotent stem cells and committed myeloid and early lymphoid progenitors. In order to gain some insight into its mechanism of action, we set out to purify and characterize its cell surface receptor. To monitor this purification, an assay capable of detecting detergent solubilized mIL-3R's was devised. With this assay, a simple two step purification protocol, involving anti-phosphotyrosine Sepharose and biotinylated mIL-3/streptavidin agarose, was developed to purify the mIL-3R This protocol was based, in part, on a previous observation in our laboratory that the mIL-3R becomes tyrosine phosphorylated upon ligand binding. Two formsof the recep.